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. 2020 Apr 8;10:6080. doi: 10.1038/s41598-020-63165-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene structures and amino acid sequence analyses of BrPAP and BrCRTISO. (a) The coding region of BrPAP includes two exons and one intron. The nonsynonymous SNP mutation (T → C) in exon 2 results in the amino acid residue conversion (Leu → Pro) between yellow-flowered and white-flowered lines. (b) The coding region of BrCRTISO contains 13 exons and 12 introns. The nonsynonymous SNP mutation (A → G) in exon 2 and (C → T) in exon 6 and a large insertion in exon 13 cause the conversion of Ile to Val and Leu to Phe, and many amino acid residue changes, respectively, between yellow-flowered and white-flowered lines. The above amino acid residue mutations are consistent with flower color phenotypes. Black backgrounds indicate mutant amino acid residues. 92S105 and 09Q5 are yellow-flowered lines; 15S1040, 15S1001, and 17S690 are white-flowered lines.