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. 2020 Apr 8;11(4):224. doi: 10.1038/s41419-020-2415-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Bioluminescent imaging (a) and quantification of photon flux (b) of lung metastatic tissues from nude mice with intravenous injection of the indicated cells (P = 0.0254). The color bars in (a) represent bioluminescent signal in radiance (p/sec/cm2/sr). c H&E staining of the dissected lungs from the nude mice in the indicated group are presented (n = 6 mice per group), showing that ASPP1 shRNA/HCT116 cells gave rise to more and larger metastases than did control ones (P = 0.0069) (d). Scale bar: 2 mm.