Fig. 5. S166 phosphorylation is essential for RIPK1-dependent skin inflammation in Sharpin^cpdm/cpdm mice.

a Kaplan-Meier curve of lesion-free survival of mice of the indicated genotypes. b Representative macroscopic pictures of mice of the indicated genotypes at the age of 15–17 weeks. c Representative skin sections from mice of the indicated genotypes at the age of 15-17 weeks stained with H&E or immunostained with antibodies against cleaved caspase-3 (CC3), keratin 6 (K6), keratin 10 (K10), keratin 14 (K14), F4/80 and DAPI (DNA stain). Scale bars in H&E and CC3 pictures 50 μm, in K6, K10/K14 and F4/80 pictures 20 μm. d Graphs depicting relative mRNA expression of the indicated genes in RNA from whole skin tissue of mice of the indicated genotypes at 15–17 weeks of age, measured by qRT-PCR. The dots in the graphs represent individual mice. Mean ± s.e.m. is shown for each group of mice in all graphs. Statistical significance was determined using a Kruskal-Wallis test (one-sided). e Representative images of H&E stained tissue sections from liver and lung from mice of the indicated genotypes at 15–17 weeks. Scale bars, 100 μm. Source data for a, d are provided as a source data file.