Fig. 2. C. elegans exposure to P. vranovensis can have multigenerational effects.

a Percent of wild-type animals surviving on plates seeded with bacterial isolate BIGb446 after 24 h. Data presented as mean values ± s.d. n = 3 experiments of >100 animals. b Percent of wild-type animals surviving on plates seeded with bacterial isolate BIGb446 after 24 h. Data presented as mean values ± s.d. n = 3–6 experiments of 500 animals each. Wild-type animals carried the integrated transgene nIs470. c Graphical representation of multigenerational experimental set up. See Fig. 1a for legend. Embryos were collected by bleaching (E.C.B.) in each generation and were placed onto fresh plates seeded with E. coli HB101 until the L4 developmental stage at each generation until the final generation, where animals were placed onto fresh plates seeded with P. vranovensis BIGb446 and the percent of surviving animals was counted after 24 h. Source data are provided as a Source Data file. ***p = 0.003, ****p < 0.0001. See section “statistics and reproducibility” for statistical tests run.