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. 2020 Apr 8;11(4):225. doi: 10.1038/s41419-020-2422-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A1 The immunohistochemical (IHC) staining of ZNF703 expressionin 128 pairs of HCC tissues and adjacent nontumor tissues Scale bars: 100 μm (low magnification) and 50 μm (high magnification); A2 Immunofluorescentimages of HCCLM3 and SMMC7721 cells stained for ZNF703 (green) and 4′, 6-diamidino-2-phenylindole (DAPI; blue) Scale bars: 50 μm. B Real-time PCR analysis of ZNF703 mRNA expression in 20 pairs of HCC specimens (T) and adjacent nontumor tissues (N). C Real-time PCR and western blotting analysis of ZNF703 level in the LO2 cell line and seven HCC cell lines. D Representative protein expression of ZNF703 in HCC tissues (T) and adjacent nontumor tissues (N) were detected by western blotting analysis. E Kaplan–Meier analysis of the correlation between ZNF703 expression and overall survival or recurrence of 128 pairs HCC patients. F The prognostic value of ZNF703 was confirmed in TCGA cohort. The log-rank test was used to calculate P-values. *P < 0.05.