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. 2020 Apr 8;10:6055. doi: 10.1038/s41598-020-63209-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential requirement for Abo1 in H3K9me2 and H3K9me3, and silencing of gene expression in subtelomeric and pericentromeric regions. Bar diagrams showing the ChIP and RT-qPCR results. The Hu2185 and Hu2318 strains were used. (A) Analysis of four genes located in subtelomeric regions: H3K9me2 (left panels), H3K9me3 ChIP-qPCR (middle panels), and RT-qPCR (right panels). (B) Analysis of dh repeats located in pericentromeric regions and the Tel1L (tlh1) region: H3K9me2 ChIP-qPCR (left panels) and RT-qPCR (right panels). All qPCR experiments were performed in at least three independent experiments. Error bars indicate standard error of the mean. *p < 0.1, **p < 0.05, ***p < 0.001, two-tailed unpaired t-test. N.S., not significant (i.e., p > 0.1).