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. 2020 Apr 8;10:6065. doi: 10.1038/s41598-020-63139-9

Figure 7.

Figure 7

Effect of green tea catechins (GTCs) on the expression of urea cycle genes in mouse liver cells. Primary liver cells were cultured in DMEM (foetal bovine serum-, arginine-, and leucine-free) for 24 h in the presence or absence of GTCs (0, 10, 100 µM). Total RNA was extracted, and the mRNA expression of Nags, Cps1, Otc, Ass1, Asl, and Arg1 were examined by qRT-PCR and normalised to the expression of β-actin. Data are presented as mean ± s.e.m.; n = 4. *P < 0.05, **P < 0.01, ***P < 0.001 (ANOVA with Dunnett’s multiple comparison test).