Skip to main content
. 2020 Apr 8;11(4):227. doi: 10.1038/s41419-020-2416-1

Fig. 2. Overexpression of HOTAIRM1 inhibited tumor growth in vitro and in vivo.

Fig. 2

a High levels of HOTAIRM1 expression in B-CPAP and TPC-1 cells, achieved by transfection with pcDNA3.1-HOTAIRM1, were measured by qRT-PCR (n = 3, **p < 0.001). b, c The effect of HOTAIRM1 on PTC cell proliferation determined by CCK-8 and colony-formation assay (n = 3, **p < 0.001); d Apoptosis was evaluated by flow cytometry (n = 3, **p < 0.001). e The tumor growth curve of xenograft tumors formed from B-CPAP cells, with or without HOTAIRM1 overexpression, in the indicated week (n = 5, **p < 0.001). f Photographs of xenograft tumors after sacrifice. g The weight of xenograft tumors after sacrifice (n = 5, **p < 0.001). h Expression of HOTAIRM1 in xenograft tumors, assessed by qRT-PCR (n = 5, **p < 0.001). i Representative photographs of immunohistochemical staining of Ki-67 in xenograft tumors. Scale bar: 50 μm.