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. 2020 Apr 8;11(4):227. doi: 10.1038/s41419-020-2416-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Relative miR-107 expression levels determined by qRT-PCR in PTC samples (n = 96, **p < 0.001). b Negative correlation between HOTAIRM1 and miR-107 levels in 96 paired PTC tissues. c, d Relative expression levels of miR-107 in PTC cell lines (NPA87, CGTHW-1, K1, B-CPAP, and TPC-1) compared in a pairwise manner with expression levels in a normal human thyroid follicular epithelial cell line (Nthy-ori 3-1, n = 3, **p < 0.001). Negative correlations between HOTAIRM1 and miR-107 in the same panel of cell lines. e, f Relatively low miR-107 expression levels detected by qRT-PCR in HOTAIRM1-overexpressing PTC cells (n = 3, **p < 0.001) and in HOTAIRM1-overexpressing xenograft tumor tissues (n = 5, **p < 0.001). g Online bioinformatics software tools predicted a putative binding site between HOTAIRM1 and miR-107 and the binding site was mutated for dual-luciferase reporter assays. h A dual-luciferase reporter gene assay showed that miR-107 decreased the luciferase activity in the HOTAIRM1-WT group (n = 3, **p < 0.001). i RNA immunoprecipitation assay using an anti-Ago2 antibody and an IgG control showed a high degree of HOTAIRM1 enrichment (n = 3, **p < 0.001).