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. 2020 Apr 8;10:6083. doi: 10.1038/s41598-020-63180-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in circulating HPV-DNA levels (copies/ml) in four metastatic patients during treatment. Disease was assessed using whole-body CT scans with SD denoting stable radiological disease and PD denoting radiological progressive disease according to RECIST 1.1 criteria with specific tumour response evaluation measurements as below. (A) Patient #B005: SD was defined by 0% change to sum of diameters of target lesions according to RECIST 1.1. The patient received programmed cell death-1 (PD-1) inhibitor immunotherapy. (B) Patient #B010: PD was defined by the appearance of a new target lesion according to RECIST 1.1. Patient received treatment of radiation to the primary base of tongue cancer. C) Patient #B017: SD was defined as a 9% increase in the sum of diameter of target lesions according to RECIST 1.1. The patient received PD-1 inhibitor based immunotherapy. (D) Patient #B023: SD was defined by a 17% increase in the sum of diameters of the target lesions according to RECIST 1.1. The patient received PD-L1 inhibitor immunotherapy.