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. 2020 Apr 8;10:6072. doi: 10.1038/s41598-020-62997-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis and quantification of migration patterns can help to study cell migration mechanisms. First, a migration experiment is performed, which involves altering the migrating cells (genetic or chemical modification, choosing cells of a different type), altering the extracellular matrix (ECM), or adding chemical signal to the extracellular environment. Next, cell migration patterns are analyzed and quantified. Finally, one or more quantitative measures derived from these patterns are statistically compared between conditions to detect significant differences.