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. 2020 Jan 14;36(4):346–358. doi: 10.1007/s12264-019-00459-5

Fig. 5.

Fig. 5

HG blocks autophagic flux and inhibits Parkin/PINK1-mediated mitophagy in PC12 cells. A, B Western blots and analyses for LC3, p62, PINK1, and Parkin after HG treatment for 48 h. C qPCR analysis of PINK1 mRNA. D Western blots of the cytosolic protein α-tubulin and mitochondrial protein COX IV cytosolic (Cyto) and mitochondrial (Mito) fractions. E Western blots and analyses for Parkin and p62 in the mitochondrial fraction. COX IV served as a loading control for mitochondrial protein. *P < 0.05, **P < 0.01, ***P < 0.001 vs controls; #P < 0.05 vs HG group; n = 3.