Skip to main content
. 2020 Apr 2;11:399. doi: 10.3389/fphar.2020.00399

Figure 2.

Figure 2

Chrysin-treated CD34+ cells sustained in vitro multiple-differentiation potential and long-term hematopoietic capacity in culture. (A) Colonies derived from multipotent progenitor cells (BFU-E, burst-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte/macrophage; CFU-E, colony-forming unit-erythrocyte; CFU-GEMMs, colony-forming unit-granulocyte/erythrocyte/macrophage/megakaryocyte) after 14 days in H4434 methylcellulose culture (n = 4). (B) Representative morphological images of CFU colonies described in (A). (C) Frequency of long-term repopulating cells within Con (DMSO 0.01%), SR1 (1 µM), or C2968 (2.5 µM) cultures measured by LDA. Positive well was defined as having more than one cobblestone-like primitive hematopoietic area. All data represent the means ± SD. Compared with control unless specified, *p < 0.05 by two-tailed unpaired t-test.