FIGURE 2.

Riboflavin-induced tryptophan fluorescence quenching in A1M. (A) Fluorescence spectra of A1M between 320 and 400 nm. Increasing amounts of riboflavin (0.625, 1.25, 2.5, 5, 10, and 20 μM) was added to a solution of A1M (2.2 μM = 0.05 mg/ml) followed by fluorescence measurements using a Jasco J-810 spectropolarimeter. (B) Peak fluorescence intensity of the same concentrations of riboflavin and A1M as in (A).