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. 2020 Apr 2;11:389. doi: 10.3389/fphar.2020.00389

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Copyright © 2020 Sun, Zeng, Wang, Jia, Wu, Yu and Mao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological evaluation of the in vivo inhibition of titanium particle-induced bone loss and proinflammatory cytokine expression by magnoflorine. (A–D) Representative images of H&E (x50 and x100) and TRAP (x100 and x200) staining indicated the decreased osteolytic region and TRAP-positive OCs in the magnoflorine-treated groups. (E, F) Representative images of IHC staining for TNF-α and IL-1β. Histomorphometric analyses of the numbers of TRAP‐positive osteoclasts (G), OcS/BS (H), and numbers of TNF-α-positive (I) and IL-1β-positive (J) cells in the calvarial bone sections were conducted. The data are presented as the mean ± SD (**P < 0.01, ***P < 0.001).