Figure 4.

Ptk7 mRNA expression. RNA was converted by reverse transcription (RT), and the number of copies per 100 ng of RNA was determined in thymocytes of age-matched controls (•) and coxsackievirus (CV) B4-infected mice by using a serial dilution of a reference plasmid. CV-B4 10G (▾): thymus sampled from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus sampled from mice born to dams inoculated with CV-B4 at day 17 of gestation. The Mann–Whitney test was used for statistical analysis. n = 6–8 per group. ***p < 0.001, **p < 0.01, *p < 0.05. Data were log-transformed before analysis.