Figure 5.

Viral load in infected tissues. Thymocytes and splenocytes were sampled from fetuses at day 17 of gestation (day 17G) and from newborns at days 1 and 5 from birth, following an infection at day 10 or 17 of gestation (day 10G ▾ or 17G♦). Total RNA was extracted, converted to cDNA, and the level of viral RNA in the thymocytes (A) or splenocytes (B) was determined by qPCR and expressed as copy number per 100 ng of total RNA. n = 5–9 per group. Coxsackievirus (CV) B4 10G (▾): thymus sampled from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus sampled from mice born to dams inoculated with CV-B4 at day 17 of gestation. Data were log-transformed before analysis.