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. 2020 Apr 2;14:223. doi: 10.3389/fnins.2020.00223

FIGURE 2.

FIGURE 2

Effects of Vinp on astrocytic apoptosis after OGD/R. (A) Immunostaining showed the expression of Bcl-2 and caspase-3 in astrocytes after OGD/R. (B) Immunostaining showed the expression of Bcl-2 and caspase-3 in astrocytes after OGD/R. The nuclei were counterstained with DAPI. Scale bars = 50 μm. (C) Representative flow cytometry images of apoptosis observed with Annexin V FITC/PI staining. (D) Apoptosis analysis calculated as a percentage relative to total cells. Data are shown as the mean ± SEM (n = 3 in each group). ***P < 0.001 using one-way ANOVA followed by Tukey’s post hoc tests. Vinp: Vinpocetine; LY: LY294002; OGD/R: oxygen-glucose deprivation/reoxygenation.