Table 5. Comparison and validation of gene expression for 6 genes calculated by RNA-Seq and qPCR in bovine skin.
| Gene symbol | Amplicon size (bp) | qPCR | RNA-Seq | ||
|---|---|---|---|---|---|
| Mean (transcript copy number) | Log2 transformed transcript copy number | Mean (RPKM) | Log2 RPKM | ||
| ALOX2 | 80 | 28.32 | 4.823749 | 22.03 | 4.461398 |
| TDO2B | 95 | 28.26 | 4.82069 | 18.22 | 4.187457 |
| SYT4 | 72 | 32.44 | 5.019702 | 30.90 | 4.949535 |
| CSN2 | 75 | 28.47 | 4.831371 | 26.31 | 4.717539 |
| RPS23 | 90 | 16.82 | 4.072106 | 23.20 | 4.536053 |
| CES1 | 97 | 32.20 | 5.008989 | 29.09 | 4.862451 |
qPCR, quantitative PCR.