FIG 5.

(A) A total ion chromatograms (ES− mode, linked axis) of Aspergillus nidulans isolates A4, SP260548, and MO80069. Known metabolites are annotated as follows: 1, neoaustinone/austinolide; 2, austinol; 3, dehydroaustinol; 4, emericellamide C/D; 5, emericellamide A; 6, emericellamide E/F; 8, C₁₅H₂₆O₄ (269.1758); 9, C₂₀H₁₆O₈ (383.0769); 10, C₂₀H₂₀O₈ (387.1084); 11, C₂₀H₁₈O₈ (385.0927); 12, C₂₈H₃₂O₁₂ (559.1819); 13, C₂₇H₃₂O₉/C₂₈H₂₈O₅N₄ (499.1973). Red numbers above peaks indicate that the corresponding metabolite is unique to a single isolate. (B) The relative abundances of four known secondary metabolites produced by A. nidulans isolates were determined from the mean peak area of three biological replicates. Error bars represent ±1 standard deviation. P values were calculated for results from the clinical isolates in comparison to those for the A4 isolate (*, P < 0.05; **, P < 0.01; ***, P < 0.001; n.d., not detected).