Figure 6.

Mice treated with MSCs had fewer neutrophils but more alveolar macrophages in the lung after infection. Mice treated with MSCs or CTX were infected with Hi and sacrificed as described above. BALF cells were collected for analyses. (a) The number of BALF CD45⁺ cells (leukocytes). (b, c) Alveolar macrophages (CD11b⁻CD11c⁺) and (d, e) neutrophils (CD11b⁺Gr-1⁺) were determined by surface staining, and both their percentages of leukocytes and absolute numbers were shown. (f, g) Tregs in the lungs were determined by intracellular staining. Data were expressed as the means ± SEM. n = 4 mice for each treatment group; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ns: no significant difference. This experiment is representative of three individual experiments.