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. 2020 Apr 5;2020:8978930. doi: 10.1155/2020/8978930

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Copyright © 2020 Xiaocheng Cao et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of DNMT1 overexpression on stem-like features of MHCC97H cells. (a) DNMT1 protein amounts in MHCC97H cells transfected with Lent-DNMT1, with β-actin as a loading control. (b) miR-34a-5p levels in MHCC97H cells transfected with Lent-DNMT1. (c) miR-34a promoter methylation in MHCC97H cells transfected with Lent-DNMT1. (d, e) Representative images of spheres and colonies in MHCC97H cells transfected with Lent-DNMT1 (left) (scale bar, 200 μm); sphere formation efficiencies and colony formation rates (right). (f) CD133 expression in MHCC97H cells transfected with DNMT1 cDNA. (g) CD44 protein expression in MHCC97H cells transfected with Lent-DNMT1. (h) Bmi1, Sox2, and Oct4 mRNA amounts in MHCC97H cells transfected with Lent-DNMT1. ^∗P < 0.05 vs. MHCC97H cells (n = 3);^#P < 0.05 vs. MHCC97H cells transfected with Lent-NC.