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. 2020 Apr 7;2020:3908641. doi: 10.1155/2020/3908641

Figure 8.

Figure 8

Aggravation of OGD/R-induced cell injury by Hsp70 knockdown was blocked by p38 MAPK inhibition. (a) shRNA-Hsp70 further reduced cell viability during OGD/R, which was abolished by SB203580 (n = 8). (b) Increase in LDH release by shRNA-Hsp70 was blocked by SB203580 (n = 8). (c) shRNA-Hsp70 further increased [Ca2+]i level during OGD/R, which was inhibited by SB203580 (n = 5). (d and e) shRNA-Hsp70 further activated IL-1β and IL-6 mRNA expression, which was blocked by SB203580 (n = 5). (f) Representative TUNEL staining images. Scale bar = 50 μm. (g) shRNA-Hsp70 further increased cell apoptosis during OGD/R, which was reversed by SB203580 (n = 5). Data are shown as means ± SEM. ∗P < 0.05, ∗∗P < 0.01 vs. the NC group; #P < 0.05, ##P < 0.01 vs. the NC + OGD/R group; &&P < 0.01 vs. the sh − Hsp70 + OGD/R group. NC: negative control transfection; OGD/R: oxygen-glucose deprivation/reoxygenation.