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. 2020 Mar 2;10(3):88. doi: 10.3390/metabo10030088

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell and extracellular vesicles (EV) pellets were re-suspended in a Radioimmunoprecipitation assay (RIPA) buffer and subjected to SDS-PAGE and Western blot analysis. Samples were transferred to nitrocellulose membranes and probed with anti-CD9 antibody (1:2000, Abcam) followed by detection using goat anti-rabbit IgG-HRP conjugated secondary antibody. The signals were detected using an enhanced chemiluminescent (ECL) kit.