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Immunofluorescent detection of hepatocytes in a hepatocyte—nonparenchymal cell co-culture after 48 h culturing with a chicken specific, fluorescein isothiocyanate (FITC) coupled anti-albumin antibody (40× magnification, bar = 40 µm). Blue colour indicates cell nuclei with DAPI staining, while green colour refers to hepatocytes detected with the FITC conjugated antibody.
