Table 3.

Design and test results of Plackett–Burman.

Run Order	X1 Time (h)	X2 Temperature (°C)	X3 Inoculum Level	X4 Rotate Speed (rpm)	X5 pH	Optical Density
1	50	37.5	1.25	120	7.5	1.77
2	40	30	1.25	150	7.5	1.66
3	50	37.5	1	150	6	1.839
4	50	37.5	1	150	7.5	1.899
5	50	30	1.25	150	6	1.609
6	40	37.5	1.25	120	7.5	1.599
7	40	30	1	120	6	1.589
8	50	30	1.25	120	6	1.678
9	50	30	1	120	7.5	1.899
10	40	37.5	1	120	6	1.709
11	40	37.5	1.25	150	6	1.649
12	40	30	1	150	7.5	1.729