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(a) Image of avidin-coated non-fluorescent polystyrene microparticles at the bright-field mode of the microscope. (b) Image of biotin-conjugated fluorescent microbeads under fluorescence illumination. (c) Image of avidin-biotin microbead complexes at both fluorescence and bright-field modes. (scale bar = 100 μm) (d) Schematic diagram of the binding of biotin-labeled microbeads to a streptavidin-coated microparticle.
