Table 1.
List of primers for reproductive system genes analysis.
| Gene | Accession | Primers | Size | Product (bp) |
|---|---|---|---|---|
| Actb | NM_007393.5 | F: 5’-CTGACCGAGCGTGGCTACAG-3’ R: 5’-CAGTGGCCATCTCCTGCTCG-3’ |
20 | 112 |
| Gnrh | NM_008145.3 | F: 5′-CTGGTCCTATGGGTTGCGCC-3′ R: 5′-AACGGGGCCAGTGGACAGTA-3′ |
20 | 134 |
| Gnrhr | NM-014715.2 | F: 5′-GTATGCTGGAGAGTACTCTGCA-3′ R: 5′-GGATGATGAAGAGGCAGCTGAAG-3′ |
20 | 380 |
| Lhb | NM_008497.2 | F: 5′-TGCTGAGCCCAAGTGTGGTG-3′ R: 5′-AGGCACAGGAGGCAAAGCAG-3′ |
20 | 185 |
| Lhr | NM_013582.2 | F: 5′-TGCAGTGCAGCTGGCTTCTT-3′ R: 5′-CTGCTGACACCCACAAGGGG-3′ |
20 | 206 |
| Fshb | NM_008045.3 | F: 5′-GACCCAGCTCGGCCCAATAC-3′ R: 5′-CTCACGGTGCAGTCAGTGCT-3′ |
20 | 170 |
| Fshr | NM_013523.3 | F: 5′-TACAGCTCTGCCATGCTGCC-3′ R: 5′-GCGTTGAGTACGAGGAGGGC-3′ |
20 | 175 |