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. 2020 Mar 15;11(3):308. doi: 10.3390/mi11030308

Figure 6.

Figure 6

OT-mediated relocation of MB-SB from a µwP to a µws array for bacteria growth under LB-agar patch. (a-i) Fluorescence image of µws array with mCherry-expressing bacteria on MB at 0 h, (a-ii) and at 10 h. (a-iii) Brightfield image of µws array with mCherry-expressing bacteria on MB. (b-i) Fluorescence image of µws array with GFP-expressing bacteria on MB at 0 h, (b-ii) and at 10 h. (b-iii) Brightfield image of µws array with GFP-expressing bacteria on MB. Single MB carrying mCherry-expressing (a) and GFP-expressing (b) bacteria were relocated to a µws array (a-i and b-i, respectively), and incubated under a LB-agar patch for proliferation. Fluorescence (ii) and brightfield (iii) images taken after 10 h demonstrated successful, confluent bacterial growth in both the relocated mCherry- and GFP-expressing bacteria. The white dots seen in (a-iii) and (b-iii) are the MBs, from relocated MB-SB, under brightfield.