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. 2020 Feb 27;10(3):384. doi: 10.3390/ani10030384

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of alpha lipoic acid on serum testosterone levels in chickens exposed to heat stress. (A) Serum testosterone concentration was determined using a commercial ELISA kit. (B) Western blotting was used to analyze the expression of the key steroidogenic-related proteins, StAR and 3β-HSD. (C) StAR and (D) 3β-HSD protein expression levels were normalized to β-actin levels. The statistical analysis results are shown as bar graphs. The data are presented as the mean ± SE of three independent experiments. Note: * p < 0.05 vs. HS group; ^# p < 0.05 vs. C group.