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Comparison of CRISPR-Cas9 and CRISPR-Cas13a
| Item | CRISPR-Cas9 | CRISPR-Cas13a |
|---|---|---|
| Subtype | Type II | Type IV |
| Guide RNA | tracrRNA and crRNA | crRNA |
| Target substrate preference | PAM | PFS |
| Nuclease domain | HNH and RuvC | HEPN |
| crRNA processing | RNase III | Helical-1 domain |
| Applications | DNA targeting, tracking, editing RNA targeting, tracking | DNA detection RNA targeting, tracking |
