Figure 5.

Growth curves of the wild-type and mutant of K. pneumoniae strains. No significant differences were observed among the parental K. pneumoniae strain Kp36 and its derivative mutants (Kp36-117R, Kp36 ΔwcaJ, and Kp36-117R pwcaJ) after 2 h. Optical densities (OD₆₀₀) were measured at 1 h intervals over an 8 h period of incubation at 37 °C in LB broth with aeration. For strain Kp36-117R pwcaJ, media was supplemented with apramycin (100 μg/mL) and 0.4% L-arabinose. Error bars represent standard errors from the experiment performed in triplicate.