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. 2020 Mar 11;8(3):389. doi: 10.3390/microorganisms8030389

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Electrophoretic and immunoblot analysis of C. jejuni ΔcadF transformants. Bacterial whole-cell lysates were separated in SDS 12.5% polyacrylamide gels and either: (A) stained with Coomassie Brilliant Blue R-250 or (B) transferred to polyvinylidene fluoride membranes and probed with a rabbit α-CadF serum. The positive control consisted of a C. jejuni wild-type strain, and the negative controls consisted of a mutant with a cadF gene deletion (ΔcadF) and a mutant with a promoterless cadF gene fused to a FLAG-tag (ΔcadF::cadF_NP-FLAG). The arrow indicates the CadF protein. Lanes: C. jejuni strains (1) 81-176 (wild-type); (2) ΔcadF; (3) ΔcadF::cadF_NP-FLAG; (4) ΔcadF::P_cadF_{-283 bp} cadF-FLAG; (5) ΔcadF::P_cadF_{-334 bp} cadF-FLAG; and (6) ΔcadF::P_cadF_{-400 bp} cadF-FLAG. Molecular mass standards (in kDa) are on the left.