Skip to main content
. 2020 Mar 11;8(3):389. doi: 10.3390/microorganisms8030389

Figure 5.

Figure 5

C. jejuni adherence to INT 407 cells requires both CadF and FlpA. In vitro adherence assays were performed with human INT 407 epithelial cells and the C. jejuni 81-176 wild-type strain, C. jejuni ΔcadF and ΔflpA mutants, and complemented strains as outlined in the “Materials and Methods” section. (A) Transformation of a C. jejuni ΔcadF mutant (ΔcadF::cadFNP-FLAG) but not the ΔcadF ΔflpA mutant restores binding to the level observed with the wild-type strain. (B) Transformation of a C. jejuni flpA deletion mutant (ΔflpA) but not the ΔcadF ΔflpA mutant restores binding to the level observed with the wild-type strain. Values represent the number of adherent bacteria ± standard deviation. The asterisks indicate that the number of bacteria bound to the INT 407 cells was statistically different (* p < 0.05) from that of the C. jejuni wild-type strain, as judged by one-way ANOVA followed by Dunnett’s multiple comparisons test.