Table 1.
C. jejuni isolates used in this study.
| Isolate | Description | Plasmid Used to Generate the Isolate | Antibiotic Resistance 1 | Reference |
|---|---|---|---|---|
| 81-176 | Wild-type strain | None | TetR | [23] |
| ΔflgL | flgL deletion mutant | pBSK-Kan2-flgL-CAT | TetR CmR | [24] |
| ΔcadF | cadF deletion mutant | pBSK-Kan2-cadF-CAT | TetR CmR | This study |
| ΔflpA | flpA deletion mutant | pBSK-flpA-Kan2 | TetR KanR | This study |
| ΔcadF ΔflpA | cadF flpA double deletion mutant | pBSK-flpA-Kan2 | TetR CmR KanR | This study |
| ΔcadF::cadFNP-FLAG | cadF deletion mutant with promoterless cadF gene fused to a FLAG-tag | prRNA-HygroR-cadFNP-FLAG | TetR CmR HygroR | This study |
| ΔcadF::PcadF-400 bp cadF-FLAG | cadF deletion mutant harboring a wild-type copy of cadF | prRNA-SpecR-PcadF cadF400 bp | TetR CmR HygroR SpecR | This study |
| ΔcadF::PcadF-334 bp cadF-FLAG | cadF deletion mutant harboring a wild-type copy of cadF | prRNA-SpecR-PcadF cadF334 bp | TetR CmR HygroR SpecR | This study |
| ΔcadF::PcadF-283 bp cadF-FLAG | cadF deletion mutant harboring a wild-type copy of cadF | prRNA-SpecR-PcadF cadF283 bp | TetR CmR HygroR SpecR | This study |
| ΔflpA::PflpA flpA | flpA deletion mutant harboring a wild-type copy of flpA | prRNA-HygroR-PflpA flpA | TetR KanR HygroR | This study |
| ΔcadF ΔflpA::cadFNP-FLAG | cadF flpA double deletion mutant with a promoterless cadF gene fused to a FLAG-tag | prRNA-HygroR-cadFNP-FLAG | TetR CmR KanR HygroR | This study |
| ΔcadF ΔflpA::PcadF-400 bp cadF-FLAG | cadF flpA double deletion mutant harboring a wild-type copy of cadF | prRNA-SpecR-PcadF cadF400 bp | TetR CmR KanR HygroR SpecR | This study |
| ΔcadF ΔflpA::PcadF-334 bp cadF-FLAG | cadF flpA double deletion mutant harboring a wild-type copy of cadF | prRNA-SpecR-PcadF cadF334 bp | TetR CmR KanR HygroR SpecR | This study |
| ΔcadF ΔflpA::PcadF-283 bp cadF-FLAG | cadF flpA double deletion mutant harboring a wild-type copy of cadF | prRNA-SpecR-PcadF cadF283 bp | TetR CmR KanR HygroR SpecR | This study |
| ΔcadF ΔflpA::PflpA flpA | cadF flpA double deletion mutant harboring a wild-type copy of flpA | prRNA-HygroR-PflpA flpA | TetR CmR KanR HygroR | This study |
| ΔcadF ΔflpA::PcadF cadF-FLAG-PflpA flpA | cadF flpA double deletion mutant harboring a wild-type copies of cadF and flpA | prRNA-SpecR-PflpA flpA-PcadF cadF400 bp | TetR CmR KanR HygroR SpecR | This study |
| 81-176 ciaD-ACD | Wild-type strain harboring ciaD-ACD fusion vector | pRY111-HygroR-PcysM ciaD-ACD | TetR HygroR | This study |
| ΔcadF ciaD-ACD | cadF deletion mutant harboring ciaD-ACD fusion vector | pRY111-HygroR-PcysM ciaD-ACD | TetR CmR HygroR | This study |
| ΔflpA ciaD-ACD | flpA deletion mutant harboring ciaD-ACD fusion vector | pRY111-HygroR-PcysM ciaD-ACD | TetR KanR HygroR | This study |
| ΔcadF ΔflpA ciaD-ACD | cadF flpA double deletion mutant harboring ciaD-ACD fusion vector | pRY111-HygroR-PcysM ciaD-ACD | TetR CmR KanR HygroR | This study |
| ΔflgL ciaD-ACD | flgL deletion mutant harboring ciaD-ACD fusion vector | pRY111-HygroR-PcysM ciaD-ACD | TetR CmR HygroR | This study |
1 CmR: Chloramphenicol; HygroR: Hygromycin; KanR: Kanamycin; SpecR: Spectinomycin; TetR: Tetracycline.