Table 2.

Bacterial plasmids used in this study.

Plasmid	Description	Antibiotic Resistance 1	Reference
pBSK-Kan2	pBlueScript II SK (+) cloning vector with the aphA-3 gene cassette encoding KanR (Kan2) replacing the original ampicillin resistance cassette	KanR	[25]
pBSK-Kan2-cadF-CAT	pBSK-Kan2 suicide plasmid harboring 5′ and 3′ cadF flanking fragments with a chloramphenicol resistance cassette (CAT)	CmR KanR	This study
pBSK-flpA-Kan2	pBSK suicide plasmid harboring 5′ and 3′ flpA flanking fragments with the C. jejuni kanamycin resistance gene (aphA-3, Kan2)	AmpR KanR	This study
prRNA-HygroR	pBSK-Kan2 vector harboring the 16S rRNA and the 23S rRNA gene fragments with a hygromycin B resistance cassette	KanR HygroR	[26]
prRNA-HygroR-cadFNP-FLAG	prRNA-HygroR suicide vector harboring the promoterless cadF gene fused with a FLAG-tag	KanR HygroR	This study
prRNA-SpecR-PcadF cadF400 bp	prRNA-SpecR suicide vector harboring 400 bp of the cadF gene at the 5′ end with its native promoter	KanR SpecR	This study
prRNA-SpecR-PcadF cadF334 bp	prRNA-SpecR suicide vector harboring 334 bp of the cadF gene at the 5′ end with its native promoter	KanR SpecR	This study
prRNA-SpecR-PcadF cadF283 bp	prRNA-SpecR suicide vector harboring 283 bp of the cadF gene at the 5′ end with its native promoter	KanR SpecR	This study
prRNA-HygroR-PflpA flpA	prRNA- HygroR suicide vector harboring the flpA gene with its native promoter	KanR HygroR	This study
prRNA-SpecR-PflpA flpA-PcadF cadF400 bp	prRNA-SpecR suicide vector harboring the flpA gene and 400 bp of the cadF gene at the 5′ end with their native promoters	KanR SpecR	This study
pRY111-HygroR-PcysM ciaD-ACD	pRY111-HygroR shuttle vector harboring the ciaD gene with a C. jejuni cysM promoter and fused with the adenylate cyclase domain (ACD) coding sequence	HygroR	This study

¹ Amp^R: Ampicillin; Cm^R: Chloramphenicol; Hygro^R: Hygromycin; Kan^R: Kanamycin; Spec^R: Spectinomycin.