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(a,b) The schematic design of the immobilization system. The narrowed channel had 25 μm depth with 55 μm width. The depth in the middle of the narrowed channel with length of 100 μm was increased to 65 μm where called as “injection area”. (c) The microinjector channels composed of four channels: Loading channel, needle channel, immobilization channel and unloading channel.
