. 2020 Mar 3;10(3):421. doi: 10.3390/ani10030421

Table 4.

Sperm quality parameters (mean ± S.E.M.) measured in turkey semen frozen in the presence of sucrose according to the different concentrations and two dilution rates (N = 8).

Semen Treatment		Sperm Parameters
NP-CPA Concentration	Dilution rate	MOT. %	MOT. PROG. %	VCL (µm/s)	VSL (µm/s)	VAP (µm/s)	LIN (%)	STR (%)	Membrane Integrity (%)	Osmotic Resistance (%)	DNA Integrity (%)
50 mM	1:2	24.2 ± 1.7^a	1.8 ± 0.3^a	30.1 ± 1.5^a	7.7 ± 0.7^a	14.3 ± 1.1^a	23.5 ± 1.6^abc	46.3 ± 1.0^b-e	31.0 ± 1.3^ab	18.1 ± 1.8^abc	97.0 ± 0.3^ab
100 mM	1:2	19.6 ± 1.9^b	1.3 ± 0.4^ab	28.3 ± 2.8^a	5.8 ± 0.7^ab	12.0 ± 1.0^ab	18.6 ± 1.5^c	41.9 ± 1.5^e	31.2 ± 1.4^ab	17.5 ± 1.7^abc	96.5 ± 0.2^bc
200 mM	1:2	22.1 ± 1.7^ab	1.2 ± 0.3^ab	28.3 ± 2.1^a	6.8 ± 0.9^a	12.4 ± 1.1^ab	24.0 ± 2.1^abc	46.1± 1.3^c-e	29.2 ± 2.0^b	17.0 ± 1.3^abc	96.0 ± 0.3^cd
400 mM	1:2	13.0 ± 0.4^c	0.7 ± 0.2^c	21.2 ± 1.3^b	4.5 ± 0.4^b	8.2 ± 0.4^c	20.6 ± 2.0^bc	47.4 ± 1.4^a	22.3 ± 1.4^d	14.7 ± 0.9^c	95.5 ± 0.2^d
50 mM	1:4	20.8 ± 1.0^ab	1.3 ± 0.2^ab	27.6 ± 1.3^a	6.6 ± 0.4^ab	13.2 ± 0.8^a	24.7 ± 2.0^abc	44.2 ± 1.0^c-e	28.5 ± 2.1^bc	19.2 ± 1.3^ab	96.9 ± 0.3^ab
100 mM	1:4	22.9 ± 1.1^ab	1.6 ± 0.3^a	28.5 ± 1.6^a	7.3 ± 0.8^a	13.6 ± 1.1^a	27.0 ± 2.9^ab	46.7 ± 2.2^ab	34.7 ± 1.2^a	20.7 ± 1.8^a	97.3 ± 0.2^a
200 mM	1:4	20.5 ± 1.2^ab	1.4 ± 0.1^ab	27.4 ± 1.5^a	7.6 ± 0.5^a	13.3 ± 0.8^a	29.9 ± 1.5^a	48.5 ± 1.1^a	31.3 ± 0.8^ab	19.8 ± 0.8^ab	96.5 ± 0.2^bc
400 mM	1:4	15.8 ± 1.0^c	0.6 ± 0.2^c	21.5 ± 1.0^b	6.2 ± 1.0^ab	10.2 ± 1.1^bc	24.3 ± 4.2^abc	47.6 ± 2.5^a	24.8 ± 1.3^cd	16.2 ± 0.5^bc	96.5 ± 0.1^bc
Concentration effect		p = 0.000	p = 0.002	p = 0.000	p = 0.035	p = 0.000	p = 0.258	p = 0.146	p = 0.000	p = 0.034	p = 0.000
Dilution rate effect		p = 0.755	p = 0.941	p = 0.498	p = 0.215	p = 0.156	p = 0.258	p = 0.009	p = 0.187	p = 0.027	p = 0.080
Concentration × dilution		p = 0.129	p = 0.463	p = 0.787	p = 0.363	p = 0.212	p = 0.182	p = 0.524	p = 0.201	p = 0.848	p = 0.053

^a–e Values within a column reporting different superscript letter differ significantly at p < 0.05. MOT: total motility; MOT PROG: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN: linearity; STR: straightness.