. 2020 Mar 3;10(3):421. doi: 10.3390/ani10030421

Table 5.

Sperm quality parameters (mean ± S.E.M.) outcomes in turkey semen frozen with trehalose considering different concentrations and two dilution rates (N = 8).

Semen Treatment		Sperm Parameters
NP-CPA Concentration	Dilution rate	MOT. %	MOT. PROG. %	VCL (µm/s)	VSL (µm/s)	VAP (µm/s)	LIN (%)	STR (%)	Membrane Integrity (%)	Osmotic Resistance (%)	DNA Integrity (%)
50 mM	1:2	21.1 ± 2.2^ab	1.6 ± 0.5^ab	29.4 ± 2.5^a	6.8 ± 0.9^abc	13.2 ± 1.6^ab	20.9 ± 2.0^ab	44.3 ± 1.1^ab	29.2 ± 1.7^ab	16.8 ± 1.4^a	96.8 ± 0.2^ab
100 mM	1:2	18.7 ± 1.7^bc	1.5 ± 0.3^ab	27.9 ± 2.4^a	5.4 ± 0.9^ab	10.9 ± 1.5^abc	18.1 ± 1.6^b	42.2 ± 0.9^ab	30.6 ± 1.9^a	18.5 ± 1.4^a	96.9 ± 0.1^ab
200 mM	1:2	16.7 ± 1.6^cd	0.9 ± 0.1^bc	23.7 ± 1.2^a	4.9 ± 0.6^a	9.8 ± 0.9^bc	17.7 ± 2.0^b	40.9 ± 1.7^b	27.5 ± 1.8^ab	16.9 ± 1.9^a	95.9 ± 0.3^c
400 mM	1:2	13.3 ± 0.9^d	0.5 ± 0.1^c	20.0 ± 1.5^b	4.3 ± 0.7^b	8.1 ± 1.1^c	18.1 ± 2.4^b	45.6 ± 1.7^ab	21.9 ± 1.8^c	17.1 ± 1.3^a	95.1 ± 0.5^d
50 mM	1:4	21.6 ± 1.2^ab	1.5 ± 0.2^ab	29.2 ± 1.8^a	7.6 ± 0.7^ab	13.7 ± 1.1^a	26.0 ± 2.9^a	46.3 ± 2.1^ab	31.9 ± 1.1^a	19.1 ± 1.3^a	96.9 ± 0.2^ab
100 mM	1:4	23.5 ± 1.1^a	1.7 ± 0.3^a	28.0 ± 1.9^a	8.0 ± 1.0^a	14.0 ± 1.4^a	28.2 ± 1.5^a	47.1 ± 0.8^a	32.0 ± 1.4^a	19.9 ± 0.6^a	97.1 ± 0.2^a
200 mM	1:4	18.6 ± 0.9^bc	1.0 ± 0.2^bc	25.4 ± 1.6^a	6.7 ± 0.7^abc	11.8 ± 0.8^abc	27.0 ± 3.0^a	47.1 ± 2.4^a	30.9 ± 1.5^a	19.1 ± 0.9^a	96.6 ± 0.2^abc
400 mM	1:4	14.2 ± 0.9^d	0.5 ± 0.1^c	24.9 ± 1.2^b	5.6 ± 0.6^abc	11.0 ± 1.1^abc	22.9 ± 2.7^ab	45.9 ± 1.9^ab	25.2 ± 1.3^bc	17.6 ± 1.4^a	96.1 ± 0.2^bc
Concentration effect		p = 0.000	p = 0.000	p = 0.002	p = 0.039	p = 0.013	p = 0.417	p = 0.613	p = 0.000	p = 0.573	p = 0.000
Dilution rate effect		p = 0.037	p = 0.999	p = 0.212	p = 0.006	p = 0.017	p = 0.000	p = 0.008	p = 0.017	p = 0.090	p = 0.009
Concentration × dilution		p = 0.366	p = 0.796	p = 0.473	p = 0.720	p = 0.711	p = 0.824	p = 0.375	p = 0.899	p = 0.894	p = 0.336

^a–d Values within a column reporting different superscript letter differ significantly at p < 0.05. MOT: total motility; MOT PROG: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN: linearity; STR: straightness.