. 2020 Mar 3;10(3):421. doi: 10.3390/ani10030421

Table 6.

Sperm quality variables (mean ± S.E.M.) obtained in turkey semen frozen in the presence of Ficoll considering different concentrations and two dilution rates (N = 8).

Semen Treatment		Sperm Parameters
NP-CPA Concentration	Dilution rate	MOT. %	MOT. PROG. %	VCL (µm/s)	VSL (µm/s)	VAP (µm/s)	LIN (%)	STR (%)	Membrane Integrity (%)	Osmotic Resistance (%)	DNA Integrity (%)
0.5 mM	1:2	26.9 ± 1.1^bcd	2.8 ± 0.3^abc	33.3 ± 1.3^ab	8.5 ± 0.5^b	15.8 ± 0.7^abc	24.0 ± 1.1^b	45.3 ± 0.9^c	34.1 ± 1.5^c	19.1 ± 2.0^a	97.4 ± 0.3^bc
0.75 mM	1:2	30.7 ± 2.2^ab	3.0 ± 0.4^ab	34.0 ± 1.3^a	8.5 ± 0.4^b	16.3 ± 0.8^abc	24.2 ± 0.9^b	44.7 ± 0.7^c	37.1 ± 1.8^bc	22.4 ± 2.4^a	97.8 ± 0.2^ab
1 mM	1:2	29.0 ± 1.6^bc	2.7 ± 0.3^abc	33.0 ± 1.3^ab	8.9 ± 0.6^b	16.2 ± 0.9^abc	24.3 ± 1.6^b	45.4 ± 0.9^c	36.0 ± 1.7^bc	21.4 ± 1.9^a	97.5 ± 0.2^abc
1.5 mM	1:2	23.5 ± 1.7^de	1.9 ± 0.3^cd	30.0 ± 1.9^ab	8.0 ± 0.9^b	14.4 ± 1.3^bc	24.2 ± 1.9^b	46.5 ± 1.3^bc	36.0 ± 1.0^bc	19.8 ± 1.5^a	96.7 ± 0.1^d
0.5 mM	1:4	25.1 ± 0.8^cde	1.8 ± 0.3^d	29.3 ± 1.4^b	7.7 ± 0.2^b	13.7 ± 0.4^c	28.5 ± 1.3^b	49.5 ± 0.9^ab	37.1 ± 1.2^bc	23.1 ± 1.7^a	97.1 ± 0.1^cd
0.75 mM	1:4	30.8 ± 0.6^ab	2.4 ± 0.2^bcd	32.8 ± 0.6^ab	8.8 ± 0.1^b	17.5 ± 1.6^ab	26.9 ± 0.8^b	48.0 ± 0.9^abc	40.5 ± 0.9^ab	23.9 ± 1.9^a	97.8 ± 0.2^ab
1 mM	1:4	32.6 ± 0.3^a	3.5 ± 0.2^a	33.9 ± 0.8^a	11.0 ± 0.6^a	18.5 ± 0.4^a	34.1 ± 2.3^a	51.6 ± 1.8^a	42.5 ± 1.2^a	24.4 ± 1.8^a	97.9 ± 0.1^a
1.5 mM	1:4	22.1 ± 1.4^e	1.6 ± 0.1^d	31.2 ± 1.2^ab	7.8 ± 0.9^b	15.5 ± 1.8^abc	27.0 ± 2.6^b	48.3 ± 1.8^abc	35.7 ± 1.9^c	22.3 ± 1.5^a	96.6 ± 0.1^d
Concentration effect		p = 0.000	p = 0.000	p = 0.059	p = 0.005	p = 0.052	p = 0.123	p = 0.431	p = 0.024	p = 0.534	p = 0.000
Dilution rate effect		p = 0.510	p = 0.198	p = 0.378	p = 0.435	p = 0.453	p = 0.000	p = 0.000	p = 0.004	p = 0.073	p = 0.735
Concentration × dilution		p = 0.034	p = 0.013	p = 0.162	p = 0.276	p = 0.099	p = 0.152	p = 0.391	p = 0.158	p = 0.931	p = 0.112

^a–e Values within a column reporting different superscript letter differ significantly at p < 0.05. MOT: total motility; MOT PROG: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN: linearity; STR: straightness.