Table 2.
List of target genes and primers sequences for RT-qPCR.
| Gene a | NCBI Gene ID | Primer Sequences (5′-3′) | Function b | Ref. |
|---|---|---|---|---|
| Panel 1. Immune-related genes | ||||
| IL-2 | 373958 | F: GCTTATGGAGCATCTCTATCATCA R: GGTGCACTCCTGGGTCTC |
Cytokine important for the proliferation of T and B lymphocytes. Important role in the immune response to antigenic stimuli. | [41] |
| IL-4 | 416330 | F: GCTCTCAGTGCCGCTGATG R: GGAAACCTCTCCCTGGATGTC |
Pleiotropic cytokine produced by activated T cells. B-cell stimulatory factor. | [43] |
| IL-6 | 395337 | F: AGGACGAGATGTGCAAGAAGTTC R: TTGGGCAGGTTGAGGTTGTT |
Cytokine that plays a role in inflammation and the maturation of B cells. Produced at sites of acute and chronic inflammation. | [44] |
| IL-10 | 428264 | F: CATGCTGCTGGGCCTGAA R: CGTCTCCTTGATCTGCTTGATG |
Pleiotropic effects in immunoregulation and inflammation. Inhibits synthesis of cytokines. | [45] |
| IL-12p40 | 404671 | F: TTGCCGAAGAGCACCAGCCG R: CGGTGTGCTCCAGGTCTTGGG |
Can act as a growth factor for activated T and Natural Killer cells. Stimulates production of IFN-gamma. | [46] |
| IL-17 | 395111 | F: GGGATTACAGGATCGATGAGGA R: GAGTTCACGCACCTGGAATG |
Cytotoxic T-lymphocyte-associated protein 8. Proinflammatory cytokine produced by activated T cells. | [41] |
| Panel 2. Stress response genes | ||||
| HSP25 | 428310 | F: CCGTCTTCTGCTGAGAGGAGTG R: ACCGTTGTTCCGTCCCATCAC |
Heat shock protein family B (small) member 9. Response to various cellular stresses. Molecular chaperones which bind to and inhibit irreversible protein aggregation or misfolding under stressful conditions. | [47] |
| HSP90AA1 | 423463 | F: GGTGTTGGTTCCTACTCTGCTTAC R: ACTGCTCATCATCATTGTGCTTGG |
Heat shock protein family class A member 1. Is a molecular chaperone that aids protein folding and quality control for a large proteins. | [47] |
| BAG3 | 423931 | F: AGGGTCGTGCGGATGTGC R: TGTGGTGGCTTAGGCTCTGC |
BAG family molecular chaperone regulator 3. Cellular response to stress. | [47] |
| CAT | 423600 | F: GGGGAGCTGTTTACTGCAAG R: CTTCCATTGGCTATGGCATT |
Catalase a key antioxidant enzyme in the bodies defense against oxidative stress. | [48] |
| SOD1 | 395938 | F: AGGGGGTCATCCACTTCC R: CCCATTTGTGTTGTCTCCAA |
Superoxide Dismutase binds copper and zinc ions. Responsible for destroying free superoxide radicals. | [48] |
| Reference genes | ||||
| ACTB | 396526 | F: CACAGATCATGTTTGAGACCTT R: CATCACAATACCAGTGGTACG |
Beta-actin is highly conserved protein involved in cell motility, structure, integrity and intercellular signaling. Ubiquitously expressed in all eukaryotic cells. | [49] |
| UB | 101747587F | F: GGGATGCAGATCTTCGTGAAA R: CTTGCCAGCAAAGATCAACCTT |
Ubiquitin is associated with protein degradation, DNA repair, cell cycle regulation kinase modification, and regulation of other cell signals pathways. | [49] |
a Annealing temperature for RT-qPCR was 58 °C except from IL-12p40 (65 °C); b gene function derive from GeneCards (http://www.genecards.org).