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. 2020 Mar 13;8(3):409. doi: 10.3390/microorganisms8030409

Figure 2.

Figure 2

Expression of pUL42 is required for nuclear transport of pUL30 in the absence of other viral proteins. MDBK cells were transiently transfected to express the indicated fusion proteins and the cell lysates were subjected to Co-IPs by using anti-HA (A) and anti-FLAG (B) antibodies. Immunoprecipitated proteins were subjected to SDS-PAGE/Western blotting assays to detect the pUL30-HA and pUL42-FLAG fusion proteins using anti-HA (upper panels) or anti-FLAG (lower panels) monoclonal antibodies. The positions of the protein markers (M) are indicated on the left. Data shown are a representative one of three independent experiments. (C) MDBK cells were transiently transfected to express the indicated fusion proteins, processed for in situ immunofluorescence and subjected to CLSM analysis using anti-HA or anti-FLAG monoclonal antibodies. The cell nuclei (DAPI) along with the pUL42-FLAG (FITC) and the pUL30-HA (TRITC) fusion proteins are shown, in grayscale, along with respective RGB merged images of the three channels (merge). RGB profile plots relative to the indicated areas were shown on the right panels (RGB profile). Images are representative of three independent transfection experiments.