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IVM impairs nuclear localization of BoHV-1 pUL42.MDBK cells were transiently transfected with pcDNA4-UL42-FLAG in the presence and absence of IVM (25 µM), processed for in situ immunofluorescence and subjected to CLSM analysis at ×63 magnification objective using an anti-FLAG monoclonal antibody. The pUL42 protein is shown in green (FITC); the nucleus is shown in blue (DAPI); and merged images of the two channels are shown on the right (merge).
