Figure 5.

IVM does not affect cell viability, virus binding and internalization. (A) MDBK cells were incubated with increasing concentrations of Ivermenctin for 48 h. Cell viability was then measured using an MTT assay. Values represent the mean± SD of the three independent experiments. (B) BoHV-1 was pretreated with various concentrations of IVM at 37°C for 1 h and incubated with MDBK cells at a MOI of 0.1 PFU/cell at 4 °C for 1 h. The unbound viruses were removed by washing cells with PBS, and the cells were incubated at 37°C for a further 48 h. (C) MDBK cells were incubated with BoHV-1 at MOI of 0.1 PFU per cell at 4 °C for 1 h; the unbound viruses were removed by washing cells with PBS. Then the cells were treated with increasing concentrations of IVM, and incubated at 37 °C for 30 min. The cells were subsequently washed with citrate buffer (pH 3.0) to remove residual viruses and cultured in maintenance media at 37 °C for 48 hrs. The virus production in (B) and (C) was determined by PRAs. Data shown are the mean ±SD from three independent experiments. Statistical significance was calculated using Student’s t test according to *p<0.05, **p<0.01, and ***p<0.001.