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. 2020 Mar 4;8(3):363. doi: 10.3390/microorganisms8030363

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western blot analysis of recombinant Cpgp40/15 and Cpgp40 in E. coli and native Cpgp40/15 and Cpgp40 in C. parvum sporozoites. (a) Expression of recombinant Cpgp40/15 and Cpgp40 in E. coli assessed by Western blot analysis using an anti-GST tag. Lane M: protein size makers. Lanes 1,2: lysate from bacterial cells transformed with pGEX-4t-1-Cpgp40/15 or pGEX-4t-1-Cpgp40 with IPTG induction, respectively. (b) Western blot analysis of native Cpgp40/15 in C. parvum sporozoites. Lane M: protein size makers. Lane 1: native proteins from sporozoites. Lane 2: proteins from human ileocecal adenocarcinoma (HCT-8) cells. (c) Western blot analysis of native Cpgp40 in C. parvum sporozoites. Lane M: protein size makers. Lane 1: native proteins from sporozoites. Lane 2: proteins from HCT-8 cells. (d) Western blot analysis of native Cpgp40/15 and Cpgp40 using pre-immune serum. Lane M: protein size makers. Lanes 1,2: native proteins from sporozoites.