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. 2020 Mar 4;8(3):363. doi: 10.3390/microorganisms8030363

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence microscopic detection of Cpgp40/15 and Cpgp40 in different C. parvum life cycle stages. (a) Distribution of Cpgp40 in the sporozoites using the rabbit anti-Cpgp40 antibody and pre-immune serum. (b) Association of Cpgp40/15 with the parasitophorous vacuole membrane (PVM) during the parasite intracellular developmental stages, including early and mature meronts, using rabbit anti-Cpgp40/15 antibody and pre-immune serum. Images were taken by differential interference contrast microscopy (DIC), fluorescence microscopy using nuclear stain 4,6-diamidino-2-phenylindole (DAPI); Scale-bars: 5 μm.