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. 2020 Mar 5;18(3):149. doi: 10.3390/md18030149

Figure 4.

Figure 4

(a) Five fractions (Fractions A, B, C, D and E) were collected from a fresh 95% ethanol extraction of P. aurata; (b) the HRMS analysis of Fraction C identified ions at m/z 236 and 469; (c) comparison of the native MRMS spectrum for free Rv1466 (top, red) with the incubation of Rv1466 with Fraction C (bottom, blue). A similar ionization pattern was observed as described in Figure 3 with Pool Fractions 4 and 5. Ions corresponding to free Rv1466 (P) and the Rv1466-ligand complex (P-L) are labeled. Molecular weight of ligand in Fraction C: MW = (2462.51133 – 2423.66863) × 6 = 233 Da.