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. 2020 Mar 20;11(3):322. doi: 10.3390/mi11030322

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Selective single-cell retrieval. (a,b) A single lymphocyte can be trapped in the hydrodynamic constriction (a) and gently released (b) through application of negative DEP force activated by 10 Vpp voltage at 10 MHz. (c,d) The cell at the top is released, while the cell at the bottom is kept in the trap. The release is carried out with a 10 Vpp voltage at 10 MHz. A custom-made printed circuit board (PCB) enables the selective release of a single T lymphocyte. For more details, please refer to the Supplementary Material Video S3.