Table 1.
Specific roe deer primer sequences used for RT-qPCR.
| Gene | Primer Sequence (5′-->3′) | PCR size (bp) | Accession Number | Reference | |
|---|---|---|---|---|---|
| VEGF121 | For: | GTTCATCTTCAAGCCGTCCTGTG | 130 | AF 152593 | Present study |
| Rev: | TTGGTGAGGTTTGATCCGCATAATC | ||||
| VEGF165 | For: | CCACCGAGGAGTTCAACATCAC | 177 | AF 152594 | Present study |
| Rev: | CAAACAAATGCTTTCTCCGCTCTG | ||||
| VEGFR1 | For: | GAGTCACGGAAGAGGATG | 171 | NM_001191132 | Present study |
| Rev: | TTAACAGGAGCCAGAAGAG | ||||
| VEGFR2 | For: | GGCTACTTCTTGTCATCGTTCTAC | 137 | NM_001110000 | Present study |
| Rev: | TCGTAAGGCAGGCGTTCAC | ||||
| GAPDH | For: | CACCGTCCATGCCATCAC | 109 | AF363637 | Present study |
| Rev: | CTCCGATGCCTGCTTCACTACCTT |
VEGF121: Vascular endothelial growth factor isoform 121; VEGF165: Vascular endothelial growth factor isoform 165; VEGFR1: Vascular endothelial growth factor receptor 1; VEGFR2: Vascular endothelial growth factor receptor 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; bp: base pair; for: forward; rev: reverse.