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Schematic representation of the methodology. Culture media abbreviations: MGIT = Mycobacteria Growth Indicator Tube; LJ = Löwenstein–Jensen; Col = Coletsos; 7H11 = Middlebrook 7H11 supplemented with oleic acid-albumin-dextrose-catalase (OADC) enrichment; 7H9a+mj = agar-solidified 7H9 medium supplemented with OADC and mycobactin J; HEYM = in-house Herrold´s Egg Yolk medium containing sodium pyruvate and mycobactin J. + = positive result; - = negative result.
